BPC-157 + TB-500 (20 mg Blend) Dosage Protocol

Quickstart Highlights

BPC-157 (Body Protection Compound-157) is a 15-amino-acid peptide fragment derived from human gastric juice, studied for its cytoprotective and tissue-repair properties across gastrointestinal, musculoskeletal, and neurological systems^[1]^[2]. TB-500 (Thymosin β4) is a 43-amino-acid peptide involved in cell migration, angiogenesis, and wound healing^[3]^[4]. This blend combines both peptides for synergistic tissue-repair research applications.

Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL total concentration (~3.33 mg/mL of each peptide).

Typical daily range: 250–500 mcg BPC-157 + 250–500 mcg TB-500 (500–1,000 mcg total blend) once daily.

Easy measuring: At 6.67 mg/mL total, 1 unit = 0.01 mL ≈ 66.7 mcg total (~33.3 mcg of each peptide) on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 14 days.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL total)

Route: Subcutaneous injection | Frequency: Once daily

Week	Daily Dose (total blend)	Per-Peptide Dose	Units (mL)
Weeks 1–2	500 mcg (0.5 mg)	~250 mcg each	7.5 units (0.075 mL)
Weeks 3–4	666 mcg (0.67 mg)	~333 mcg each	10 units (0.10 mL)
Weeks 5–8	1,000 mcg (1.0 mg)	~500 mcg each	15 units (0.15 mL)

For ≤10-unit (≤0.10 mL) administrations during Weeks 1–4, consider using 30- or 50-unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming or direct stream onto the powder.

Gently swirl or roll until fully dissolved (do not shake).

Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Advanced / Loading Phase (Optional)

Some protocols use a higher TB-500 loading dose during the first 2–4 weeks based on clinical monograph recommendations^[5]. This schedule uses higher total blend volumes.

Route: Subcutaneous injection | Frequency: Once daily or split twice weekly for TB-500 loading

Phase	Daily Dose (total blend)	Per-Peptide Dose	Units (mL)
Loading (Weeks 1–4)	1,500 mcg (1.5 mg)	~750 mcg each	22.5 units (0.225 mL)
Maintenance (Weeks 5–8)	1,000 mcg (1.0 mg)	~500 mcg each	15 units (0.15 mL)

Note: Advanced protocols consume peptide supplies faster. Plan vial quantities accordingly.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on a 4–8 week daily protocol (standard approach at ~1 mg/day maintenance).

Peptide Vials (BPC-157 + TB-500 20 mg Blend):

4 weeks (28 doses at ~0.67–1.0 mg/day): 2 vials
6 weeks (42 doses): 3 vials
8 weeks (56 doses): 3–4 vials

Insulin Syringes (U-100):

Per week: 7 syringes (1/day)
4 weeks: 28 syringes
6 weeks: 42 syringes
8 weeks: 56 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

4 weeks (2 vials): 6 mL → 1 × 10 mL bottle
6 weeks (3 vials): 9 mL → 1 × 10 mL bottle
8 weeks (4 vials): 12 mL → 2 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
4 weeks: 56 swabs → recommend 1 × 100-count box
6 weeks: 84 swabs → recommend 1 × 100-count box
8 weeks: 112 swabs → recommend 2 × 100-count boxes

View Supplier

Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support tissue repair, wound healing, and musculoskeletal recovery in research contexts^[1]^[3].

Schedule: Daily subcutaneous injections for 4–8 weeks; some protocols include 3-month-on / 6-week-off cycling.

Dose Range: 500–1,500 mcg total blend daily (providing ~250–750 mcg of each peptide).

Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL total) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; use within 14 days^[6].

Research Disclaimer

BPC157 + TB500 (20mg Blend Vial) Dosage Protocol