TB-500 (5mg Vial) & BPC-157 (5mg Vial) Dosage Protocol

TB-500 + BPC-157 Stack (5 mg Vials) Dosage Protocol

Quickstart Highlights

TB-500 (Thymosin β4) is a 43-amino-acid peptide that promotes tissue repair and regeneration through enhanced angiogenesis and cellular migration[1][2]. BPC-157 (Body Protection Compound-157) is a 15-amino-acid gastric peptide studied for accelerating musculoskeletal healing by upregulating growth factor signaling[3][4]. Stacking these peptides is a common research approach for synergistic tissue repair support.

  • TB-500 Reconstitution: Add 2.0 mL bacteriostatic water → 2.5 mg/mL concentration.
  • BPC-157 Reconstitution: Add 2.0 mL bacteriostatic water → 2.5 mg/mL concentration.
  • TB-500 Dosing: 1,250 mcg (50 units) twice weekly (Weeks 1–4), then once weekly (Weeks 5–12).
  • BPC-157 Dosing: 250–500 mcg (10–20 units) daily, titrated over 12 weeks.
  • Easy measuring: At 2.5 mg/mL, 1 unit = 0.01 mL = 25 mcg on a U-100 insulin syringe.
  • Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate and use within ~30 days.

    • Dosing & Reconstitution Guide

    Educational guide for reconstitution and combined dosing protocol

    TB-500 Protocol (2 mL Reconstitution = 2.5 mg/mL)

    Week Dose (mcg) Units (per injection) (mL) Frequency
    Weeks 1–4 1,250 mcg (1.25 mg) 50 units (0.50 mL) Twice weekly
    Weeks 5–12 1,250 mcg (1.25 mg) 50 units (0.50 mL) Once weekly

    Route: Subcutaneous injection. The loading phase (Weeks 1–4) uses twice-weekly dosing to establish tissue saturation, followed by a maintenance phase (Weeks 5–12) at once weekly[1].

    TB-500 Reconstitution Steps

  • Draw 2.0 mL bacteriostatic water with a sterile syringe.
  • Inject slowly down the vial wall; avoid foaming.
  • Gently swirl/roll until dissolved (do not shake).
  • Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

    • BPC-157 Protocol (2 mL Reconstitution = 2.5 mg/mL)

    Week Daily Dose (mcg) Units (per injection) (mL) Frequency
    Weeks 1–2 250 mcg (0.25 mg) 10 units (0.10 mL) Once daily
    Weeks 3–8 500 mcg (0.50 mg) 20 units (0.20 mL) Once daily
    Weeks 9–12 250 mcg (0.25 mg) 10 units (0.10 mL) Once daily

    Route: Subcutaneous injection, preferably near the site of injury. Preclinical research commonly uses 250–500 mcg per day for musculoskeletal applications[3][4]. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.

    BPC-157 Reconstitution Steps

  • Draw 2.0 mL bacteriostatic water with a sterile syringe.
  • Inject slowly down the vial wall; avoid foaming.
  • Gently swirl/roll until dissolved (do not shake).
  • Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

    • Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

    Supplies Needed

    Plan based on an 8–16 week combined protocol with the schedules above.

  • TB-500 Vials (5 mg each):

    • 8 weeks ≈ 3 vials
    • 12 weeks ≈ 4 vials
    • 16 weeks ≈ 5 vials
  • BPC-157 Vials (5 mg each):

    • 8 weeks ≈ 5 vials
    • 12 weeks ≈ 7 vials
    • 16 weeks ≈ 8 vials
  • Insulin Syringes (U-100):

    • 8 weeks: 68 syringes (TB-500: 12 + BPC-157: 56)
    • 12 weeks: 100 syringes (TB-500: 16 + BPC-157: 84)
    • 16 weeks: 132 syringes (TB-500: 20 + BPC-157: 112)
  • Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.

    • 8 weeks (8 total vials): 16 mL → 2 × 10 mL bottles
    • 12 weeks (11 total vials): 22 mL → 3 × 10 mL bottles
    • 16 weeks (13 total vials): 26 mL → 3 × 10 mL bottles
  • Alcohol Swabs: One for the vial stopper + one for the injection site each administration.

    • 8 weeks: ~160 swabs → recommend 2 × 100-count boxes
    • 12 weeks: ~232 swabs → recommend 3 × 100-count boxes
    • 16 weeks: ~304 swabs → recommend 4 × 100-count boxes
    • TB-500 5mg Vial

    View TB-500

    BPC-157 5mg Vial

    View BPC-157

    Insulin Syringes

    View Supplier

    Bacteriostatic Water

    View Supplier

    Alcohol Pads

    View Supplier

    Protocol Overview

    Concise summary of the combined regimen.

  • Goal: Support tissue repair, reduce inflammation, and accelerate recovery from musculoskeletal injuries[1][3].
  • Schedule: TB-500 twice weekly (loading) then once weekly (maintenance); BPC-157 daily throughout.
  • Dose Range: TB-500: 1,250 mcg per injection; BPC-157: 250–500 mcg daily.
  • Reconstitution: 2.0 mL per 5 mg vial for both peptides (~2.5 mg/mL).
  • Storage: Refrigerate lyophilized and reconstituted solutions; use within ~30 days after reconstitution.