DSIP (5 mg Vial) Dosage Protocol

Quickstart Highlights

Delta Sleep-Inducing Peptide (DSIP) is a naturally occurring nonapeptide (9 amino acids) first isolated from rabbit brain tissue and studied for its effects on sleep architecture and stress modulation^[1]^[2]. Research indicates DSIP may promote delta-wave (slow-wave) sleep, modulate cortisol and ACTH levels, and exhibit stress-protective properties^[3]^[4]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration.

Typical daily range: 100–300 mcg once daily (gradual titration); advanced up to 500 mcg.

Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~1.67 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Week 1	100 mcg	6 units (0.06 mL)
Week 2	150 mcg	9 units (0.09 mL)
Week 3	200 mcg	12 units (0.12 mL)
Weeks 4–8	250–300 mcg	15–18 units (0.15–0.18 mL)

Frequency: Inject once daily subcutaneously, typically in the evening before bedtime^[5]^[6]. For ≤10-unit (≤0.10 mL) administrations (Weeks 1–2), consider 30- or 50-unit insulin syringes for improved readability.

Advanced / Extended Approach

Week	Daily Dose (mcg)	Units (per injection) (mL)
Week 5	350–400 mcg	21–24 units (0.21–0.24 mL)
Weeks 6–8+	400–500 mcg	24–30 units (0.24–0.30 mL)

Note: Advanced dosing (beyond 300 mcg) is based on anecdotal experience; formal human studies have primarily used doses up to ~300 mcg daily^[7]. Use the minimum effective dose and increase only if needed.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–12 week daily protocol with gradual titration.

Peptide Vials (DSIP, 5 mg each):

8 weeks at ~200 mcg/day avg ≈ 2–3 vials
12 weeks at ~200 mcg/day avg ≈ 3–4 vials
12 weeks at ~300 mcg/day avg ≈ 5–6 vials

Insulin Syringes (U-100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

3 vials: 9 mL → 1 × 10 mL bottle
5 vials: 15 mL → 2 × 10 mL bottles
6 vials: 18 mL → 2 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes

View Supplier

Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support healthy sleep architecture and stress modulation over time^[3]^[4].

Schedule: Daily subcutaneous injections for 4–8 weeks (extend to 12 weeks if desired).

Dose Range: 100–300 mcg daily with gradual titration; advanced up to 500 mcg.

Reconstitution: 3.0 mL per 5 mg vial (~1.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

Research Disclaimer

DSIP (5mg Vial) Dosage Protocol