NAD+ (500 mg Vial) Dosage Protocol

Quickstart Highlights

NAD+ (nicotinamide adenine dinucleotide) is a critical coenzyme involved in cellular energy metabolism, DNA repair, and mitochondrial function^[1]. Clinical research has primarily employed intravenous infusions at high doses, though subcutaneous administration at lower doses is emerging as a practical maintenance route^[2]^[3]. This educational protocol presents a once‑daily subcutaneous approach with gradual titration for improved tolerability.

Reconstitute: Add 3.0 mL bacteriostatic water → 166.7 mg/mL concentration.

Typical daily range: 50–100 mg once daily subcutaneously (gradual titration from lower doses).

Easy measuring: At 166.7 mg/mL, 1 unit = 0.01 mL = 1.67 mg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 14 days; protect from light and avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Titration Approach (3 mL = 166.7 mg/mL)

Week	Daily Dose (mg)	Units (per injection) (mL)
Week 1	50 mg	30 units (0.30 mL)
Week 2	75 mg	45 units (0.45 mL)
Weeks 3–8	100 mg	60 units (0.60 mL)
Weeks 9–12	100 mg	60 units (0.60 mL)
Weeks 13–16	100 mg	60 units (0.60 mL)

Frequency: Inject once daily subcutaneously. This gradual titration protocol begins at 50 mg daily to assess tolerance, as starting too high can produce adverse reactions such as insomnia, anxiety, or fatigue^[4]. Most individuals find doses in the 50–100 mg range well‑tolerated after ramp‑up^[5]. The 3.0 mL dilution keeps per‑injection volumes practical and allows for accurate unit measurements on standard insulin syringes.

Reconstitution Steps

Allow the lyophilized vial to reach room temperature before opening to minimize moisture condensation.

Draw 3.0 mL bacteriostatic water (0.9% benzyl alcohol) with a sterile syringe.

Inject slowly down the vial wall to avoid foaming; do not aim directly at the powder.

Gently swirl or roll the vial until the powder fully dissolves (do not shake vigorously).

The resulting solution should be clear and colorless. If discoloration or precipitate appears, discard.

Label with the reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Use within 14 days; inspect before each use for clarity.

Note: Each 0.01 mL (1 unit) contains approximately 1.67 mg of NAD+. Example conversions: 50 mg = 30 units; 75 mg = 45 units; 100 mg = 60 units.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration as outlined above.

Peptide Vials (NAD+, 500 mg each):

8 weeks ≈ 11 vials (total 5,075 mg used)
12 weeks ≈ 16 vials (total 7,875 mg used)
16 weeks ≈ 22 vials (total 10,675 mg used)

Insulin Syringes (U‑100, 1 mL):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

8 weeks (11 vials): 33 mL → 4 × 10 mL bottles
12 weeks (16 vials): 48 mL → 5 × 10 mL bottles
16 weeks (22 vials): 66 mL → 7 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily subcutaneous regimen.

Goal: Support cellular energy metabolism, DNA repair pathways, and mitochondrial function through exogenous NAD+ supplementation^[1].

Schedule: Daily subcutaneous injections for 8–16 weeks with gradual dose titration.

Dose Range: 50–100 mg daily; start low (50 mg) and increase by ~25 mg weekly as tolerated.

Reconstitution: 3.0 mL per 500 mg vial (166.7 mg/mL) for accurate unit measurements.

Storage: Lyophilized powder frozen at −20 °C (−4 °F); reconstituted solution refrigerated at 2–8 °C (35.6–46.4 °F) for up to 14 days; protect from light.

Research Disclaimer