TB-500 (10 mg Vial) Dosage Protocol

Quickstart Highlights

TB‑500 is a synthetic peptide fragment corresponding to the active region of thymosin beta‑4 (Tβ4), a naturally occurring 43‑amino‑acid protein involved in tissue repair and regeneration^[1]^[2]. This educational protocol presents a once‑daily subcutaneous approach using a practical dilution for accurate insulin‑syringe measurements in research settings.

Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.

Typical daily range: 500–1000 mcg once daily (gradual titration recommended).

Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); do not freeze reconstituted solution.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~3.33 mg/mL)

Phase	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	500 mcg	15 units (0.15 mL)
Weeks 3–4	600 mcg	18 units (0.18 mL)
Weeks 5–8	750 mcg	23 units (0.23 mL)
Weeks 9–12	1000 mcg	30 units (0.30 mL)

Frequency: Inject once daily subcutaneously. This schedule uses the largest practical dilution (3.0 mL) to keep per‑injection units in a comfortable range for accurate measurement. Total weekly dose averages ~5 mg, consistent with research protocols^[3]^[4].

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label with date and concentration; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. TB‑500 is not FDA‑approved for human use and is for research purposes only.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (TB‑500, 10 mg each):

8 weeks ≈ 4 vials
12 weeks ≈ 7 vials
16 weeks ≈ 10 vials

Insulin Syringes (U‑100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (4 vials): 12 mL → 2 × 10 mL bottles
12 weeks (7 vials): 21 mL → 3 × 10 mL bottles
16 weeks (10 vials): 30 mL → 3 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

View Supplier

Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support tissue repair, wound healing, and angiogenesis through the active thymosin beta‑4 fragment mechanism^[5]^[6].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if research goals require).

Dose Range: 500–1000 mcg daily with gradual titration (~5 mg/week average).

Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid freeze–thaw cycles of reconstituted solution.

Research Disclaimer