5-Amino-1MQ (50 mg Vial) Dosage Protocol

Quickstart Highlights

5-Amino-1MQ dosage protocols center on this selective, cell-permeable NNMT (Nicotinamide N-methyltransferase) inhibitor studied for its potential to support fat metabolism, preserve lean muscle mass, and elevate intracellular NAD+ levels^[1]^[2]. By blocking NNMT, 5-Amino-1MQ may help restore cellular energy balance and activate SIRT1 pathways associated with metabolic efficiency^[3]. This educational protocol presents a subcutaneous injection approach to maximize bioavailability from the 50 mg vial format.

Reconstitute: Add 4.0 mL bacteriostatic water → 12.5 mg/mL concentration.

Typical daily range: 2.5–5 mg once or twice daily (subcutaneous).

Easy measuring: At 12.5 mg/mL, 1 unit = 0.01 mL = 0.125 mg (125 mcg) on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (4 mL = 12.5 mg/mL)

Phase	Daily Dose (mg)	Units (per injection) (mL)
Days 1–2 (Tolerance)	2.5 mg once daily	20 units (0.20 mL)
Days 3+ (Standard)	5 mg once daily	40 units (0.40 mL)
Alternative BID	2.5 mg twice daily	20 units (0.20 mL) × 2

Frequency: Inject once or twice daily subcutaneously. Due to the compound’s plasma half-life of approximately 3.8–6.9 hours^[4], twice-daily (BID) dosing may provide more sustained NNMT inhibition. A single 50 mg vial provides 10–20 days of research material at these doses.

Reconstitution Steps

Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.

Draw 4.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved—solution should be clear (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on research duration using the 50 mg vial format at 5 mg/day.

5-Amino-1MQ Vials (50 mg each):

1 week (5 mg/day) ≈ 1 vial
2 weeks (5 mg/day) ≈ 1 vial
4 weeks (5 mg/day) ≈ 2 vials

Insulin Syringes (U-100):

Per week (once daily): 7 syringes
Per week (BID): 14 syringes
4 weeks (once daily): 28 syringes

Bacteriostatic Water (10 mL bottles): Use 4.0 mL per vial for reconstitution.

1 week (1 vial): 4 mL → 1 × 10 mL bottle
2 weeks (1 vial): 4 mL → 1 × 10 mL bottle
4 weeks (2 vials): 8 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each administration.

Per week (once daily): 14 swabs
4 weeks (once daily): 56 swabs → recommend 1 × 100-count box

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Protocol Overview

Concise summary of the subcutaneous regimen.

Goal: Support metabolic efficiency through NNMT inhibition, potentially enhancing fat oxidation and NAD+ levels^[1]^[2].

Schedule: Daily subcutaneous injections; each 50 mg vial provides 10–20 days of research material.

Dose Range: 2.5–5 mg once or twice daily.

Reconstitution: 4.0 mL per 50 mg vial (12.5 mg/mL) for accurate measurements.

Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F).