AICAR (50 mg Vial) Dosage Protocol

Quickstart Highlights

AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) is a research compound that activates AMP-activated protein kinase (AMPK)^[1], earning the nickname “exercise pill” after studies showed sedentary animals given AICAR experienced dramatic endurance improvements^[2]. AICAR is prohibited in sports (WADA classifies it as a metabolic modulator) and is not approved for therapeutic use in humans^[1]. This educational protocol presents a once-daily subcutaneous approach for research purposes only.

Reconstitute: Add 3.0 mL bacteriostatic water → ~16.7 mg/mL concentration.

Research dosing range: 1,000–3,000 mcg once daily (conservative gradual approach).

Easy measuring: At 16.7 mg/mL, 1 unit = 0.01 mL ≈ 167 mcg on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 4 weeks; avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing (research use only)

Standard / Gradual Approach (3 mL = ~16.7 mg/mL)

Week/Phase	Daily Dose	Units (per injection) (mL)
Weeks 1–2	1,000 mcg (1 mg)	6 units (0.06 mL)
Weeks 3–4	2,000 mcg (2 mg)	12 units (0.12 mL)
Weeks 5–8	3,000 mcg (3 mg)	18 units (0.18 mL)

Frequency: Inject once daily subcutaneously^[3]. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Advanced Protocol (Higher Doses)

Week/Phase	Daily Dose	Units (per injection) (mL)
Weeks 1–2	2,000 mcg (2 mg)	12 units (0.12 mL)
Weeks 3–6	3,000 mcg (3 mg)	18 units (0.18 mL)
Weeks 7–12	5,000 mcg (5 mg)	30 units (0.30 mL)

Note: Higher-dose protocols should only be considered when explicitly supported by literature^[4]. Research doses remain well below human safety studies (up to 210 mg/kg IV)^[5], providing a wide safety margin.

Important: This guide is for educational and research purposes only and is not medical advice. Not for human consumption.

Supplies Needed

Plan based on an 8-week research protocol (conservative dosing).

Peptide Vials (AICAR, 50 mg each):

8 weeks (gradual 1–3 mg/day) ≈ 3 vials
12 weeks (2–5 mg/day advanced) ≈ 8 vials

Insulin Syringes (U-100, or 30/50-unit for precision):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

8 weeks (3 vials): 9 mL → 1 × 10 mL bottle
12 weeks (8 vials): 24 mL → 3 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes

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Protocol Overview

Concise summary of the once-daily research regimen.

Research Goal: Activate AMPK pathways to mimic exercise-like metabolic effects^[1]^[6].

Schedule: Daily subcutaneous injections for 8–12 weeks (research protocols).

Dose Range: 1,000–3,000 mcg daily (conservative); up to 5,000 mcg in advanced protocols.

Reconstitution: 3.0 mL per 50 mg vial (~16.7 mg/mL) for precise measurements.

Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated 2–8 °C (35.6–46.4 °F) for up to 4 weeks^[14].