Cardiogen (20 mg Vial) Dosage Protocol

Quickstart Highlights

Cardiogen is a synthetic cardiovascular bioregulator tetrapeptide (AEDR: Ala‑Glu‑Asp‑Arg) studied for its cellular repair and cardioprotective properties^[1]^[2]. Preclinical research demonstrates Cardiogen enters cells and modulates fundamental survival pathways, supporting cytoskeletal integrity and reducing apoptosis in stressed cardiac tissue while paradoxically encouraging apoptosis in abnormal cells^[3]^[4]. This educational protocol presents a once‑daily subcutaneous approach for research applications.

Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.

Typical daily range: 200–500 mcg once daily (gradual titration over 8–16 weeks).

Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	200 mcg	3 units (0.03 mL)
Weeks 3–4	300 mcg	4.5 units (0.045 mL)
Week 5	400 mcg	6 units (0.06 mL)
Weeks 6–12	500 mcg	7.5 units (0.075 mL)

Frequency: Inject once daily subcutaneously. This schedule uses the largest practical dilution (3.0 mL) to maximize measurement precision. For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (Cardiogen, 20 mg each):

8 weeks ≈ 2 vials
12 weeks ≈ 2 vials
16 weeks ≈ 3 vials

Insulin Syringes (U‑100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (2 vials): 6 mL → 1 × 10 mL bottle
12 weeks (2 vials): 6 mL → 1 × 10 mL bottle
16 weeks (3 vials): 9 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support cellular repair mechanisms and cardiovascular tissue resilience in research models^[1]^[2].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 200–500 mcg daily with gradual titration.

Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.