Crystagen (20 mg Vial) Dosage Protocol

Quickstart Highlights

Crystagen (Glu-Asp-Pro) is a tripeptide bioregulator studied for immune system modulation and cellular regulation^[1]. This synthetic peptide is designed to support immune function and has been investigated in research settings for its immunomodulatory properties^[2]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → 6.67 mg/mL concentration.

Typical daily range: 1000–2000 mcg once daily (gradual titration to 2000 mcg).

Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U-100 insulin syringe.

Storage: Lyophilized: refrigerate at 4 °C (39.2 °F) or freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 4 °C (39.2 °F) for up to 30 days.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = 6.67 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Week 1	1000 mcg (1 mg)	15 units (0.15 mL)
Week 2	1500 mcg (1.5 mg)	23 units (0.23 mL)
Weeks 3–12	2000 mcg (2 mg)	30 units (0.30 mL)

Frequency: Inject once daily subcutaneously. This schedule uses a 3.0 mL dilution to keep per-injection units ≥10 for accurate measuring. Research protocols typically utilize daily subcutaneous injections for systemic immune modulation^[1]^[2]. Similar immunomodulatory peptides have been administered in daily injection courses in clinical settings^[3].

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 4 °C (39.2 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (Crystagen, 20 mg each):

8 weeks ≈ 6 vials
12 weeks ≈ 9 vials
16 weeks ≈ 12 vials

Insulin Syringes (U-100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (30 mL bottles): Use 3.0 mL per vial for reconstitution. Multi-use bacteriostatic water vials should be discarded 28 days after opening^[7].

8 weeks (6 vials): 18 mL → 1 × 30 mL bottle (plan for second vial if exceeding 28 days)
12 weeks (9 vials): 27 mL → 2 × 30 mL bottles
16 weeks (12 vials): 36 mL → 2–3 × 30 mL bottles (replace after ~8 weeks for sterility)

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes
16 weeks: 224 swabs → recommend 3 × 100-count boxes

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Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support immune system modulation and cellular regulation through bioregulatory peptide signaling^[1]^[2].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 1000–2000 mcg daily with gradual titration over 2 weeks.

Reconstitution: 3.0 mL per 20 mg vial (6.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized: refrigerate or freeze; reconstituted: refrigerate for up to 30 days^[5]^[6].

Research Disclaimer