GHK-Cu (100 mg Vial) Dosage Protocol

Quickstart Highlights

GHK-Cu dosage protocols utilize this naturally occurring copper-binding tripeptide (glycyl-L-histidyl-L-lysine) to support skin rejuvenation, wound healing, and tissue repair^[1]^[2]. GHK-Cu stimulates collagen and elastin production, promotes angiogenesis, and delivers powerful antioxidant and anti-inflammatory benefits^[3]^[4]. Human studies demonstrate improved skin thickness, reduced wrinkles, and enhanced dermal density with GHK-Cu application^[2]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → ~33.3 mg/mL concentration.

Typical daily range: 1–2 mg once daily (gradual titration over 12 weeks).

Easy measuring: At 33.3 mg/mL, 1 unit = 0.01 mL ≈ 333 mcg on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 30 days.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~33.3 mg/mL)

Week	Daily Dose (mg)	Units (per injection) (mL)
Weeks 1–4	1.0 mg	3 units (0.03 mL)
Weeks 5–8	1.5 mg	4.5 units (0.045 mL)
Weeks 9–12	2.0 mg	6 units (0.06 mL)

Frequency: Inject once daily subcutaneously, 5 days per week (Mon–Fri) with weekends off^[5]. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 30 days^[6]^[7].

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–12 week daily protocol (5 days/week) with gradual titration.

Peptide Vials (GHK-Cu, 100 mg each):

8 weeks (~40 injections, ~50 mg total) ≈ 1 vial
12 weeks (~60 injections, ~90 mg total) ≈ 1 vial

Insulin Syringes (U-100, 30- or 50-unit preferred):

Per week: 5 syringes (1/day, 5 days)
8 weeks: 40 syringes
12 weeks: 60 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

8–12 weeks (1 vial): 3 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 10 swabs (2/day, 5 days)
8 weeks: 80 swabs → recommend 1 × 100-count box
12 weeks: 120 swabs → recommend 2 × 100-count boxes

View Supplier

Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support skin rejuvenation, collagen synthesis, wound healing, and anti-aging tissue repair^[2]^[3].

Schedule: Daily subcutaneous injections, 5 days on / 2 days off, for 8–12 weeks.

Dose Range: 1–2 mg daily with gradual titration.

Reconstitution: 3.0 mL per 100 mg vial (~33.3 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen at −20 °C; reconstituted refrigerated; use within 30 days.