GHK-Cu (50 mg Vial) Dosage Protocol

Quickstart Highlights

GHK-Cu (glycyl-L-histidyl-L-lysine:copper complex) is a naturally occurring copper peptide with documented roles in wound healing, tissue remodeling, and skin regeneration^[1]^[2]. Research demonstrates activity in gene regulation related to collagen synthesis, antioxidant defense, and anti-inflammatory pathways^[3]^[4]. This educational protocol presents practical subcutaneous administration approaches based on clinical practice patterns.

Reconstitute: Add 3.0 mL sterile water → 16.67 mg/mL concentration.

Typical range: 1.0–2.0 mg per injection (most common protocols use 5 days/week or 3×/week).

Easy measuring: At 16.67 mg/mL, 1 unit = 0.01 mL ≈ 167 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: store at −20 °C (−4 °F) or below; reconstituted: refrigerate at 2–8 °C (35.6–46.4 °F) and use within 30 days.

Dosing & Reconstitution Guide

Educational guide for reconstitution and common dosing patterns

Standard / Conservative Approach (3 mL = 16.67 mg/mL; 5 days/week)

Week/Phase	Dose per Injection	Units (per injection) (mL)
Weeks 1–4	1.0 mg (1000 mcg)	6 units (0.06 mL)
Weeks 5–8	1.5 mg (1500 mcg)	9 units (0.09 mL)
Weeks 9–12+	2.0 mg (2000 mcg)	12 units (0.12 mL)

Frequency: Inject once daily, 5 days per week subcutaneously^[5]^[6]. This gradual titration allows assessment of individual tolerance while maintaining practical injection volumes. For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL sterile or bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall to minimize foaming.

Gently swirl or roll the vial until the peptide fully dissolves (do not shake vigorously).

Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Alternative Protocol (3 mL = 16.67 mg/mL; 3× weekly)

Week/Phase	Dose per Injection	Units (per injection) (mL)
Weeks 1–12+	2.0 mg (2000 mcg)	12 units (0.12 mL)

Frequency: Inject 2 mg three times per week (e.g., Monday/Wednesday/Friday)^[6]. This protocol maintains consistent weekly dosing (~6 mg/week) with less frequent injections.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week protocol. Calculations shown for both 5 days/week and 3×/week protocols.

Peptide Vials (GHK-Cu, 50 mg each):

5 days/week (1.0–2.0 mg/day):
8 weeks (~50 mg total) ≈ 1 vial
12 weeks (~90 mg total) ≈ 2 vials
16 weeks (~130 mg total) ≈ 3 vials
3×/week (2 mg each):
8 weeks (~48 mg) ≈ 1 vial
12 weeks (~72 mg) ≈ 2 vials
16 weeks (~96 mg) ≈ 2 vials

Insulin Syringes (U‑100, 29–31 gauge):

5 days/week:
Per week: 5 syringes
8 weeks: 40 syringes
12 weeks: 60 syringes
16 weeks: 80 syringes
3×/week:
Per week: 3 syringes
8 weeks: 24 syringes
12 weeks: 36 syringes
16 weeks: 48 syringes

Sterile or Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

1 vial protocols: 3 mL → 1 × 10 mL bottle
2 vial protocols: 6 mL → 1 × 10 mL bottle
3 vial protocols: 9 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each administration.

5 days/week:
Per week: 10 swabs (2 per injection)
8 weeks: 80 swabs → recommend 1 × 100‑count box
12 weeks: 120 swabs → recommend 2 × 100‑count boxes
16 weeks: 160 swabs → recommend 2 × 100‑count boxes
3×/week:
Per week: 6 swabs
8 weeks: 48 swabs → recommend 1 × 100‑count box
12 weeks: 72 swabs → recommend 1 × 100‑count box
16 weeks: 96 swabs → recommend 1 × 100‑count box

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Protocol Overview

Concise summary of common GHK-Cu administration patterns.

Goal: Support tissue remodeling, wound healing, and skin regeneration through documented biological pathways^[1]^[2].

Schedule: Subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 1.0–2.0 mg per injection; frequency 3–5 days per week.

Reconstitution: 3.0 mL per 50 mg vial (16.67 mg/mL) provides practical unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated and used within 30 days.

Research Disclaimer

GHK-Cu (50mg Vial) Dosage Protocol