Glutathione (600 mg Vial) Dosage Protocol

Quickstart Highlights

Glutathione dosage protocols leverage this master antioxidant tripeptide (γ-L-glutamyl-L-cysteinyl-glycine) to support cellular detoxification, reduce oxidative stress, and maintain redox balance throughout the body^[1]^[2]. As the most abundant intracellular thiol, glutathione plays a central role in neutralizing free radicals, supporting liver function, enhancing immune cell activity, and protecting against environmental toxins^[3]^[4]. This educational protocol presents a subcutaneous injection approach using a practical dilution for clear insulin-syringe measurements.

Reconstitute: Add 2.0 mL bacteriostatic water → 300 mg/mL concentration.

Typical daily range: 100–200 mg subcutaneously (gradual titration recommended).

Easy measuring: At 300 mg/mL, 1 unit = 0.01 mL = 3 mg on a U-100 insulin syringe.

Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (2 mL = 300 mg/mL)

Week	Daily Dose (mg)	Units (per injection) (mL)
Weeks 1–2	100 mg	33 units (0.33 mL)
Weeks 3–4	150 mg	50 units (0.50 mL)
Weeks 5–8	200 mg	67 units (0.67 mL)

Frequency: Inject once daily subcutaneously, or split into every-other-day dosing based on protocol goals^[5]. Some protocols utilize 200 mg 1–2 × weekly for maintenance^[6]. This schedule uses a practical 2.0 mL dilution for accurate measurements with standard insulin syringes.

Reconstitution Steps

Draw 2.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on a 4–8 week daily protocol with gradual titration.

Peptide Vials (Glutathione, 600 mg each):

4 weeks (100–150 mg/day avg) ≈ 3 vials
8 weeks (150–200 mg/day avg) ≈ 7 vials

Insulin Syringes (U-100):

Per week: 7 syringes (1/day)
4 weeks: 28 syringes
8 weeks: 56 syringes

Bacteriostatic Water (10 mL bottles): Use ~2.0 mL per vial for reconstitution.

4 weeks (3 vials): 6 mL → 1 × 10 mL bottle
8 weeks (7 vials): 14 mL → 2 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
4 weeks: 56 swabs → recommend 1 × 100-count box
8 weeks: 112 swabs → recommend 2 × 100-count boxes

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Protocol Overview

Concise summary of the subcutaneous regimen.

Goal: Support antioxidant defense, cellular detoxification, and immune function^[1]^[3].

Schedule: Daily or every-other-day subcutaneous injections for 4–8 weeks.

Dose Range: 100–200 mg daily with gradual titration.

Reconstitution: 2.0 mL per 600 mg vial (300 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

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