LL-37 (5 mg Vial) Dosage Protocol

Quickstart Highlights

LL-37 (also known as CAP-18) is a 37-amino-acid cationic antimicrobial peptide derived from the C-terminal of human cathelicidin (hCAP18)^[1]. It is the only known human cathelicidin, exhibiting broad-spectrum antibacterial activity and immune-modulating properties^[2]^[3]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → ~1.67 mg/mL concentration.

Typical daily range: 100–400 µg once daily (gradual titration).

Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 µg on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 4 weeks; avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~1.67 mg/mL)

Week	Daily Dose (µg)	Units (per injection) (mL)
Week 1	50 µg	3 units (0.03 mL)
Week 2	100 µg	6 units (0.06 mL)
Week 3	150 µg	9 units (0.09 mL)
Week 4	200 µg	12 units (0.12 mL)
Week 5	250 µg	15 units (0.15 mL)
Week 6	300 µg	18 units (0.18 mL)
Week 7	350 µg	21 units (0.21 mL)
Week 8	400 µg	24 units (0.24 mL)

Frequency: Inject once daily subcutaneously. Some protocols use a 5-days-on, 2-days-off schedule. For ≤10-unit (≤0.10 mL) administrations during early titration, consider 30- or 50-unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (LL-37, 5 mg each):

8 weeks ≈ 3 vials
12 weeks ≈ 5 vials
16 weeks ≈ 7 vials

Insulin Syringes (U-100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (3 vials): 9 mL → 1 × 10 mL bottle
12 weeks (5 vials): 15 mL → 2 × 10 mL bottles
16 weeks (7 vials): 21 mL → 3 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100-count boxes
12 weeks: 168 swabs → recommend 2 × 100-count boxes
16 weeks: 224 swabs → recommend 3 × 100-count boxes

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Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support antimicrobial defense and wound-healing processes^[4]^[5].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 50–400 µg daily with gradual titration.

Reconstitution: 3.0 mL per 5 mg vial (~1.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.