MOTS-C (10 mg Vial) Dosage Protocol

Quickstart Highlights

MOTS-c is a 16–amino-acid mitochondrial-derived peptide (MDP) that acts as a metabolic regulator, primarily through AMPK activation^[1]^[2]. Preclinical studies show it enhances insulin sensitivity, promotes fat oxidation, improves exercise capacity, and counters age-related metabolic decline^[1]^[4]. No clinical trials have been completed in humans to date^[8]. This educational protocol presents a once-daily subcutaneous approach with gradual titration.

Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.

Typical daily range: 200–1,000 mcg once daily (gradual titration over 10 weeks).

Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F) or below; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 7 days for best potency.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~3.33 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	200 mcg (0.2 mg)	6 units (0.06 mL)
Weeks 3–4	400 mcg (0.4 mg)	12 units (0.12 mL)
Weeks 5–6	600 mcg (0.6 mg)	18 units (0.18 mL)
Weeks 7–8	800 mcg (0.8 mg)	24 units (0.24 mL)
Weeks 9–10+	1,000 mcg (1.0 mg)	30 units (0.30 mL)

Frequency: Inject once daily subcutaneously^[7]. This schedule uses the largest practical dilution (3.0 mL) to maintain per‑injection accuracy. Stay at each dose level for approximately 2 weeks before increasing, and monitor for any adverse reactions^[7].

For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake vigorously).

Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Use within 7 days for optimal potency^[7].

Important: This guide is for educational purposes only and is not medical advice. MOTS-c is an experimental compound for research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–12 week daily protocol with gradual titration.

Peptide Vials (MOTS-C, 10 mg each):

8 weeks ≈ 3 vials
12 weeks ≈ 6 vials
16 weeks ≈ 9 vials

Insulin Syringes (U‑100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (3 vials): 9 mL → 1 × 10 mL bottle
12 weeks (6 vials): 18 mL → 2 × 10 mL bottles
16 weeks (9 vials): 27 mL → 3 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support metabolic homeostasis, insulin sensitivity, and age-related physical performance based on preclinical evidence^[1]^[2]^[4].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 200–1,000 mcg daily with gradual titration over 10 weeks.

Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw.

Research Disclaimer