MOTS-C (20 mg Vial) Dosage Protocol

Quickstart Highlights

MOTS-c is a 16–amino-acid mitochondrial-derived peptide (MDP) that acts as a metabolic regulator, primarily through AMPK activation^[1]^[2]. Preclinical studies show it enhances insulin sensitivity, promotes fat oxidation, improves exercise capacity, and counters age-related metabolic decline^[1]^[4]. No clinical trials have been completed in humans to date^[8]. This educational protocol presents a once-daily subcutaneous approach with gradual titration.

Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.

Typical daily range: 200–1,000 mcg once daily (gradual titration over 10 weeks).

Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F) or below; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 7 days for best potency.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	200 mcg (0.2 mg)	3 units (0.03 mL)
Weeks 3–4	400 mcg (0.4 mg)	6 units (0.06 mL)
Weeks 5–6	600 mcg (0.6 mg)	9 units (0.09 mL)
Weeks 7–8	800 mcg (0.8 mg)	12 units (0.12 mL)
Weeks 9–10+	1,000 mcg (1.0 mg)	15 units (0.15 mL)

Frequency: Inject once daily subcutaneously^[7]. Stay at each dose level for approximately 2 weeks before increasing, and monitor for any adverse reactions^[7].

For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake vigorously).

Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Use within 7 days for optimal potency^[7].

Important: This guide is for educational purposes only and is not medical advice.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (MOTS-C, 20 mg each):

8 weeks ≈ 2 vials
12 weeks ≈ 3 vials
16 weeks ≈ 5 vials

Insulin Syringes (U‑100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (2 vials): 6 mL → 1 × 10 mL bottle
12 weeks (3 vials): 9 mL → 1 × 10 mL bottle
16 weeks (5 vials): 15 mL → 2 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support metabolic homeostasis, insulin sensitivity, and age-related physical performance based on preclinical evidence^[1]^[2]^[4].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 200–1,000 mcg daily with gradual titration over 10 weeks.

Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw.