Ovagen (20 mg Vial) Dosage Protocol

Quickstart Highlights

Ovagen is a synthetic ultrashort tripeptide (Glu-Asp-Leu; EDL) studied as a bioregulator of gastrointestinal and liver tissues^[1]. In vitro studies demonstrate that EDL increases cell proliferation and modulates aging-related gene markers (p16, p21, p53, SIRT-6) in renal cell cultures^[2]. No controlled human trials exist; available data are preclinical and cell-based. This educational protocol presents a once-daily subcutaneous approach with gradual titration using microgram-level dosing.

Reconstitute: Add 2.0 mL bacteriostatic water → 10 mg/mL (10,000 μg/mL) concentration.

Typical daily range: 10–150 μg once daily (gradual titration over 16 weeks).

Easy measuring: At 10 mg/mL, 1 unit = 0.01 mL = 100 μg on a U-100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (2 mL = 10 mg/mL)

Week	Daily Dose (μg)	Units (per injection) (mL)
Weeks 1–2	10 μg (0.01 mg)	0.1 units (0.001 mL)
Weeks 3–4	20 μg (0.02 mg)	0.2 units (0.002 mL)
Weeks 5–6	50 μg (0.05 mg)	0.5 units (0.005 mL)
Weeks 7–8	100 μg (0.1 mg)	1 unit (0.01 mL)
Weeks 9–16	100–150 μg (0.1–0.15 mg)	1–1.5 units (0.01–0.015 mL)

Frequency: Inject once daily subcutaneously. Because all per-administration volumes are ≤1.5 units (≤0.015 mL), use 30-unit or 50-unit insulin syringes for improved readability and accuracy. Titrate slowly by adding 10–20 μg (0.1–0.2 units) every 1–2 weeks as tolerated^[3].

Reconstitution Steps

Draw 2.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on a 16-week daily protocol with gradual titration. One 20 mg vial provides approximately 13,510 μg total usage over 16 weeks, so a single vial is sufficient for the full course.

Peptide Vials (Ovagen, 20 mg each):

16 weeks: 1 vial (with excess remaining)

Insulin Syringes (U-100, 30-unit or 50-unit recommended):

Per week: 7 syringes (1/day)
16 weeks: 112 syringes (recommend ~120 with extras)

Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.

16 weeks (1 vial): 2 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
16 weeks: 224 swabs → recommend 3 × 100-count boxes

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Protocol Overview

Concise summary of the once-daily regimen.

Goal: Support gastrointestinal and hepatic tissue bioregulation over time^[1].

Schedule: Daily subcutaneous injections for 16 weeks with gradual titration.

Dose Range: 10–150 μg daily with slow escalation.

Reconstitution: 2.0 mL per 20 mg vial (10 mg/mL) for precise microgram measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

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