Semax (10 mg Vial) Dosage Protocol

Quickstart Highlights

Semax is a synthetic heptapeptide analog of ACTH(4–10) developed in Russia and studied primarily for cognitive enhancement and neuroprotection^[1]^[2]. While intranasal administration is most common in clinical literature, subcutaneous injection offers a convenient once‑daily alternative for research purposes^[1]^[4]. This educational protocol presents a practical SC approach using straightforward reconstitution for accurate insulin‑syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water (max vial capacity) → ~3.33 mg/mL concentration.

Typical daily range: 300–800 mcg once daily (gradual titration recommended).

Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles^[2]^[9].

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~3.33 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	300 mcg	9 units (0.09 mL)
Weeks 3–4	500 mcg	15 units (0.15 mL)
Weeks 5–6	600 mcg	18 units (0.18 mL)
Weeks 7–8	800 mcg	24 units (0.24 mL)

For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Frequency: Inject once daily subcutaneously^[1]^[4]. This schedule uses the largest practical dilution (3.0 mL) to ensure per‑injection volumes are accurate and manageable. Most human nootropic studies use intranasal dosing in the range of 400–900 mcg/day divided into multiple administrations^[2]^[3]; this SC protocol delivers comparable total daily amounts in a single injection for convenience.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake vigorously).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light^[2].

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (Semax, 10 mg each):

8 weeks ≈ 4 vials
12 weeks ≈ 6 vials
16 weeks ≈ 8 vials

Insulin Syringes (U‑100):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (4 vials): 12 mL → 2 × 10 mL bottles
12 weeks (6 vials): 18 mL → 2 × 10 mL bottles
16 weeks (8 vials): 24 mL → 3 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily SC regimen.

Goal: Support cognitive function, attention, and neuroprotective pathways studied in clinical literature^[2]^[5].

Schedule: Daily subcutaneous injections for 8 weeks (extend to 12–16 weeks with cycling if desired)^[4].

Dose Range: 300–800 mcg daily with gradual titration; aligns with typical human nootropic dosing ranges^[2]^[3].

Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; use within 30 days^[2]^[9].

Research Disclaimer