Testagen (20 mg Vial) Dosage Protocol

Quickstart Highlights

Testagen is a synthetic tetrapeptide with the sequence Lys‑Glu‑Asp‑Gly (KEDG), an anterior pituitary‑derived bioregulator studied for its role in modulating endocrine function, particularly the pituitary–gonadal axis^[1]^[2]. Preclinical research indicates effects on thyroid hormone normalization and male reproductive hormone modulation^[3]^[4]. This educational protocol presents a once‑daily subcutaneous approach using a practical dilution for clear insulin‑syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.

Typical daily range: 100–300 mcg once daily (gradual titration).

Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	100 mcg (0.1 mg)	1.5 units (0.015 mL)
Weeks 3–4	150 mcg (0.15 mg)	2.25 units (0.0225 mL)
Weeks 5–8	200 mcg (0.2 mg)	3 units (0.03 mL)
Weeks 9–12 (optional)	250–300 mcg (0.25–0.3 mg)	3.75–4.5 units (0.0375–0.045 mL)

Frequency: Inject once daily subcutaneously, consistent with preclinical study protocols^[3]^[4]. For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake)^[5].

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

Peptide Vials (Testagen, 20 mg each):

8 weeks (~14 mg used) ≈ 1 vial
12 weeks (~25 mg used) ≈ 2 vials
16 weeks (~36 mg used) ≈ 2 vials

Insulin Syringes (30‑ or 50‑unit recommended for low‑volume accuracy):

Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

8 weeks (1 vial): 3 mL → 1 × 10 mL bottle
12 weeks (2 vials): 6 mL → 1 × 10 mL bottle
16 weeks (2 vials): 6 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‑count boxes
12 weeks: 168 swabs → recommend 2 × 100‑count boxes
16 weeks: 224 swabs → recommend 3 × 100‑count boxes

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Protocol Overview

Concise summary of the once‑daily regimen.

Goal: Support endocrine function research, particularly pituitary–gonadal axis modulation^[1]^[2].

Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).

Dose Range: 100–300 mcg daily with gradual titration.

Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate unit measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

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