Vesugen (20mg Vial) Dosage Protocol

Vesugen (Lys-Glu-Asp) (20 mg Vial) Dosage Protocol

Quickstart Highlights

Vesugen (Lys-Glu-Asp, also known as KED) is a tripeptide bioregulator synthesized at the Saint Petersburg Institute of Bioregulation and Gerontology[1]. Research suggests it may support vascular endothelial cell proliferation through epigenetic regulation of Ki-67 gene expression[2]. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

  • Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.
  • Typical daily range: 500–2000 mcg once daily (gradual titration).
  • Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U-100 insulin syringe.
  • Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
    • Vesugen Vial

    Dosing & Reconstitution Guide

    Educational guide for reconstitution and daily dosing

    Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

    Week Daily Dose (mcg) Units (per injection) (mL)
    Week 1 500 mcg (0.5 mg) 7.5 units (0.075 mL)
    Week 2 1000 mcg (1.0 mg) 15 units (0.15 mL)
    Week 3 1500 mcg (1.5 mg) 22.5 units (0.225 mL)
    Weeks 4–8+ 1500–2000 mcg (1.5–2.0 mg) 22.5–30 units (0.225–0.30 mL)

    Frequency: Inject once daily subcutaneously. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability. Note that Week 1 dosing falls at 7.5 units; a 50-unit syringe with 0.5-unit graduations improves accuracy.

    Reconstitution Steps

  • Draw 3.0 mL bacteriostatic water with a sterile syringe[9].
  • Inject slowly down the vial wall; avoid foaming.
  • Gently swirl/roll until dissolved (do not shake).
  • Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light[10].

    • Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

    Supplies Needed

    Plan based on an 8–16 week daily protocol with gradual titration (targeting ~1500 mcg/day maintenance).

  • Peptide Vials (Vesugen, 20 mg each):
    • 8 weeks (~84 mg total) ≈ 5 vials
    • 12 weeks (~126 mg total) ≈ 7 vials
    • 16 weeks (~168 mg total) ≈ 9 vials
  • Insulin Syringes (U-100):
    • Per week: 7 syringes (1/day)
    • 8 weeks: 56 syringes
    • 12 weeks: 84 syringes
    • 16 weeks: 112 syringes
  • Bacteriostatic Water (10 mL bottles):Use ~3.0 mL per vial for reconstitution.
    • 8 weeks (5 vials): 15 mL → 2 × 10 mL bottles
    • 12 weeks (7 vials): 21 mL → 3 × 10 mL bottles
    • 16 weeks (9 vials): 27 mL → 3 × 10 mL bottles
  • Alcohol Swabs:One for the vial stopper + one for the injection site each day.
    • Per week: 14 swabs (2/day)
    • 8 weeks: 112 swabs → recommend 2 × 100-count boxes
    • 12 weeks: 168 swabs → recommend 2 × 100-count boxes
    • 16 weeks: 224 swabs → recommend 3 × 100-count boxes

    • Vesugen Vial
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    Insulin Syringes
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    Bacteriostatic Water
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    Alcohol Pads
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    Protocol Overview

    Concise summary of the once-daily regimen.

  • Goal: Support vascular endothelial function and cellular renewal over time[2].
  • Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
  • Dose Range: 500–2000 mcg daily with gradual titration.
  • Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate unit measurements.
  • Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.