Vilon (20 mg Vial) Dosage Protocol

Quickstart Highlights

Vilon (Lys‑Glu) is a synthetic immunoregulatory dipeptide consisting of lysine and glutamic acid residues^[1]. Preclinical research indicates it may enhance immune markers including CD5+ lymphocytes and interleukin‑2 expression in thymic and splenic cell cultures^[2]^[3]. This educational protocol presents a pulsed subcutaneous approach (5 consecutive days per 4‑week cycle) using a practical dilution for clear insulin‑syringe measurements.

Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.

Typical range: 67–670 mcg once daily for 5 consecutive days per cycle.

Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 1 week or aliquot and freeze.

Dosing & Reconstitution Guide

Educational guide for reconstitution and pulsed dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Schedule: Inject once daily subcutaneously for 5 consecutive days, then rest for the remainder of a 4‑week cycle. Repeat monthly^[4]^[5].

Phase / Cycle	Daily Dose (mcg)	Units (per injection) (mL)
Cycle 1, Day 1	67 mcg (0.067 mg)	1 unit (0.01 mL)
Cycle 1, Day 2	133 mcg (0.133 mg)	2 units (0.02 mL)
Cycle 1, Day 3	200 mcg (0.20 mg)	3 units (0.03 mL)
Cycle 1, Day 4	267 mcg (0.267 mg)	4 units (0.04 mL)
Cycle 1, Day 5	333 mcg (0.33 mg)	5 units (0.05 mL)
Cycle 2+ (Days 1–5)	333–667 mcg (0.33–0.67 mg)	5–10 units (0.05–0.10 mL)

For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Reconstitution Steps

Draw 3.0 mL bacteriostatic water with a sterile syringe.

Inject slowly down the vial wall; avoid foaming.

Gently swirl/roll until dissolved (do not shake).

Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within ~1 week or aliquot and freeze^[10].

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week pulsed protocol (5 injection days per 4‑week cycle).

Peptide Vials (Vilon, 20 mg each):

8 weeks (2 cycles, 10 injections) ≈ 1 vial
12 weeks (3 cycles, 15 injections) ≈ 1 vial
16 weeks (4 cycles, 20 injections) ≈ 1 vial

Insulin Syringes (U‑100, 30‑ or 50‑unit preferred):

Per cycle: 5 syringes (1/day × 5 days)
8 weeks: 10 syringes
12 weeks: 15 syringes
16 weeks: 20 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

8–16 weeks (1 vial): 3 mL → 1 × 10 mL bottle

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per cycle: 10 swabs (2/day × 5 days)
8 weeks: 20 swabs
12 weeks: 30 swabs
16 weeks: 40 swabs → recommend 1 × 100‑count box

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Protocol Overview

Concise summary of the pulsed regimen.

Goal: Support immune modulation and thymic function markers based on preclinical observations^[1]^[2].

Schedule: 5 consecutive days of subcutaneous injections per 4‑week cycle; repeat for 2–4 cycles.

Dose Range: 67–667 mcg daily with gradual titration during the first cycle.

Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate low‑volume measurements.

Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

Research Disclaimer

Vilon (20mg Vial) Dosage Protocol